Filter
Associated Lab
- Ahrens Lab (3) Apply Ahrens Lab filter
- Aso Lab (3) Apply Aso Lab filter
- Baker Lab (5) Apply Baker Lab filter
- Betzig Lab (8) Apply Betzig Lab filter
- Branson Lab (6) Apply Branson Lab filter
- Card Lab (1) Apply Card Lab filter
- Cardona Lab (1) Apply Cardona Lab filter
- Chklovskii Lab (2) Apply Chklovskii Lab filter
- Cui Lab (2) Apply Cui Lab filter
- Darshan Lab (1) Apply Darshan Lab filter
- Dickson Lab (6) Apply Dickson Lab filter
- Druckmann Lab (1) Apply Druckmann Lab filter
- Dudman Lab (4) Apply Dudman Lab filter
- Eddy/Rivas Lab (3) Apply Eddy/Rivas Lab filter
- Egnor Lab (1) Apply Egnor Lab filter
- Fetter Lab (1) Apply Fetter Lab filter
- Fitzgerald Lab (1) Apply Fitzgerald Lab filter
- Freeman Lab (3) Apply Freeman Lab filter
- Gonen Lab (10) Apply Gonen Lab filter
- Grigorieff Lab (3) Apply Grigorieff Lab filter
- Harris Lab (2) Apply Harris Lab filter
- Heberlein Lab (2) Apply Heberlein Lab filter
- Hermundstad Lab (2) Apply Hermundstad Lab filter
- Hess Lab (5) Apply Hess Lab filter
- Jayaraman Lab (1) Apply Jayaraman Lab filter
- Ji Lab (3) Apply Ji Lab filter
- Johnson Lab (1) Apply Johnson Lab filter
- Kainmueller Lab (2) Apply Kainmueller Lab filter
- Karpova Lab (1) Apply Karpova Lab filter
- Keller Lab (8) Apply Keller Lab filter
- Lavis Lab (7) Apply Lavis Lab filter
- Lee (Albert) Lab (4) Apply Lee (Albert) Lab filter
- Leonardo Lab (4) Apply Leonardo Lab filter
- Li Lab (1) Apply Li Lab filter
- Lippincott-Schwartz Lab (12) Apply Lippincott-Schwartz Lab filter
- Liu (Zhe) Lab (4) Apply Liu (Zhe) Lab filter
- Looger Lab (11) Apply Looger Lab filter
- Magee Lab (1) Apply Magee Lab filter
- Menon Lab (3) Apply Menon Lab filter
- Murphy Lab (1) Apply Murphy Lab filter
- Pavlopoulos Lab (2) Apply Pavlopoulos Lab filter
- Reiser Lab (2) Apply Reiser Lab filter
- Riddiford Lab (4) Apply Riddiford Lab filter
- Romani Lab (2) Apply Romani Lab filter
- Rubin Lab (9) Apply Rubin Lab filter
- Saalfeld Lab (1) Apply Saalfeld Lab filter
- Scheffer Lab (7) Apply Scheffer Lab filter
- Sgro Lab (1) Apply Sgro Lab filter
- Simpson Lab (2) Apply Simpson Lab filter
- Singer Lab (10) Apply Singer Lab filter
- Spruston Lab (1) Apply Spruston Lab filter
- Stern Lab (6) Apply Stern Lab filter
- Sternson Lab (5) Apply Sternson Lab filter
- Stringer Lab (1) Apply Stringer Lab filter
- Svoboda Lab (7) Apply Svoboda Lab filter
- Tebo Lab (2) Apply Tebo Lab filter
- Tervo Lab (1) Apply Tervo Lab filter
- Tillberg Lab (1) Apply Tillberg Lab filter
- Tjian Lab (4) Apply Tjian Lab filter
- Truman Lab (1) Apply Truman Lab filter
- Turaga Lab (1) Apply Turaga Lab filter
- Turner Lab (1) Apply Turner Lab filter
- Wang (Shaohe) Lab (1) Apply Wang (Shaohe) Lab filter
- Wu Lab (2) Apply Wu Lab filter
- Zlatic Lab (1) Apply Zlatic Lab filter
- Zuker Lab (1) Apply Zuker Lab filter
Associated Project Team
Publication Date
- December 2014 (35) Apply December 2014 filter
- November 2014 (14) Apply November 2014 filter
- October 2014 (15) Apply October 2014 filter
- September 2014 (17) Apply September 2014 filter
- August 2014 (14) Apply August 2014 filter
- July 2014 (26) Apply July 2014 filter
- June 2014 (14) Apply June 2014 filter
- May 2014 (14) Apply May 2014 filter
- April 2014 (20) Apply April 2014 filter
- March 2014 (18) Apply March 2014 filter
- February 2014 (15) Apply February 2014 filter
- January 2014 (34) Apply January 2014 filter
- Remove 2014 filter 2014
Type of Publication
236 Publications
Showing 31-40 of 236 resultsMating induces pronounced changes in female reproductive behavior, typically including a dramatic reduction in sexual receptivity. In Drosophila, postmating behavioral changes are triggered by sex peptide (SP), a male seminal fluid peptide that acts via a receptor (SPR) expressed in sensory neurons (SPSNs) of the female reproductive tract. Here, we identify second-order neurons that mediate the behavioral changes induced by SP. These SAG neurons receive synaptic input from SPSNs in the abdominal ganglion and project to the dorsal protocerebrum. Silencing SAG neurons renders virgin females unreceptive, whereas activating them increases the receptivity of females that have already mated. Physiological experiments demonstrate that SP downregulates the excitability of the SPSNs, and hence their input onto SAG neurons. These data thus provide a physiological correlate of mating status in the female central nervous system and a key entry point into the brain circuits that control sexual receptivity.
The way the hippocampus processes information and encodes memories in the form of "cell assemblies" is likely determined in part by how its circuits are wired up during development. In this issue, Xu et al. now provide new insight into how neurons arising from a single common precursor migrate to their final destination and form functionally synchronous ensembles.
A major limitation in understanding embryonic development is the lack of cell type-specific markers. Existing gene expression and marker atlases provide valuable tools, but they typically have one or more limitations: a lack of single-cell resolution; an inability to register multiple expression patterns to determine their precise relationship; an inability to be upgraded by users; an inability to compare novel patterns with the database patterns; and a lack of three-dimensional images. Here, we develop new 'atlas-builder' software that overcomes each of these limitations. A newly generated atlas is three-dimensional, allows the precise registration of an infinite number of cell type-specific markers, is searchable and is open-ended. Our software can be used to create an atlas of any tissue in any organism that contains stereotyped cell positions. We used the software to generate an 'eNeuro' atlas of the Drosophila embryonic CNS containing eight transcription factors that mark the major CNS cell types (motor neurons, glia, neurosecretory cells and interneurons). We found neuronal, but not glial, nuclei occupied stereotyped locations. We added 75 new Gal4 markers to the atlas to identify over 50% of all interneurons in the ventral CNS, and these lines allowed functional access to those interneurons for the first time. We expect the atlas-builder software to benefit a large proportion of the developmental biology community, and the eNeuro atlas to serve as a publicly accessible hub for integrating neuronal attributes - cell lineage, gene expression patterns, axon/dendrite projections, neurotransmitters--and linking them to individual neurons.
We describe a method for fully automated detection of chemical synapses in serial electron microscopy images with highly anisotropic axial and lateral resolution, such as images taken on transmission electron microscopes. Our pipeline starts from classification of the pixels based on 3D pixel features, which is followed by segmentation with an Ising model MRF and another classification step, based on object-level features. Classifiers are learned on sparse user labels; a fully annotated data subvolume is not required for training. The algorithm was validated on a set of 238 synapses in 20 serial 7197×7351 pixel images (4.5×4.5×45 nm resolution) of mouse visual cortex, manually labeled by three independent human annotators and additionally re-verified by an expert neuroscientist. The error rate of the algorithm (12% false negative, 7% false positive detections) is better than state-of-the-art, even though, unlike the state-of-the-art method, our algorithm does not require a prior segmentation of the image volume into cells. The software is based on the ilastik learning and segmentation toolkit and the vigra image processing library and is freely available on our website, along with the test data and gold standard annotations (http://www.ilastik.org/synapse-detection/sstem).
View Publication PageThe behavior of individuals determines the strength and outcome of ecological interactions, which drive population, community, and ecosystem organization. Bio-logging, such as telemetry and animal-borne imaging, provides essential individual viewpoints, tracks, and life histories, but requires capture of individuals and is often impractical to scale. Recent developments in automated image-based tracking offers opportunities to remotely quantify and understand individual behavior at scales and resolutions not previously possible, providing an essential supplement to other tracking methodologies in ecology. Automated image-based tracking should continue to advance the field of ecology by enabling better understanding of the linkages between individual and higher-level ecological processes, via high-throughput quantitative analysis of complex ecological patterns and processes across scales, including analysis of environmental drivers.
Mapping the connectivity of neurons in the brain (i.e., connectomics) is a challenging problem due to both the number of connections in even the smallest organisms and the nanometer resolution required to resolve them. Because of this, previous connectomes contain only hundreds of neurons, such as in the C.elegans connectome. Recent technological advances will unlock the mysteries of increasingly large connectomes (or partial connectomes). However, the value of these maps is limited by our ability to reason with this data and understand any underlying motifs. To aid connectome analysis, we introduce algorithms to cluster similarly-shaped neurons, where 3D neuronal shapes are represented as skeletons. In particular, we propose a novel location-sensitive clustering algorithm. We show clustering results on neurons reconstructed from the Drosophila medulla that show high-accuracy.
We describe a technique for imaging single mRNAs in living cells based on fluorescent protein (FP) complementation. We employ the high affinity interaction between the bacterial phage MS2/PP7 coat proteins and their respective RNA binding motifs as an RNA scaffold to bring two halves of a split-FP together to image single reporter mRNAs without background fluorescence.
Behavioral choices that ignore prior experience promote exploration and unpredictability but are seemingly at odds with the brain's tendency to use experience to optimize behavioral choice. Indeed, when faced with virtual competitors, primates resort to strategic counterprediction rather than to stochastic choice. Here, we show that rats also use history- and model-based strategies when faced with similar competitors but can switch to a "stochastic" mode when challenged with a competitor that they cannot defeat by counterprediction. In this mode, outcomes associated with an animal's actions are ignored, and normal engagement of anterior cingulate cortex (ACC) is suppressed. Using circuit perturbations in transgenic rats, we demonstrate that switching between strategic and stochastic behavioral modes is controlled by locus coeruleus input into ACC. Our findings suggest that, under conditions of uncertainty about environmental rules, changes in noradrenergic input alter ACC output and prevent erroneous beliefs from guiding decisions, thus enabling behavioral variation.
Modern biological research relies heavily on microscopic imaging. The advanced genetic toolkit of Drosophila makes it possible to label molecular and cellular components with unprecedented level of specificity necessitating the application of the most sophisticated imaging technologies. Imaging in Drosophila spans all scales from single molecules to the entire populations of adult organisms, from electron microscopy to live imaging of developmental processes. As the imaging approaches become more complex and ambitious, there is an increasing need for quantitative, computer-mediated image processing and analysis to make sense of the imagery. Bioimage Informatics is an emerging research field that covers all aspects of biological image analysis from data handling, through processing, to quantitative measurements, analysis and data presentation. Some of the most advanced, large scale projects, combining cutting edge imaging with complex bioimage informatics pipelines, are realized in the Drosophila research community. In this review, we discuss the current research in biological image analysis specifically relevant to the type of systems level image datasets that are uniquely available for the Drosophila model system. We focus on how state-of-the-art computer vision algorithms are impacting the ability of Drosophila researchers to analyze biological systems in space and time. We pay particular attention to how these algorithmic advances from computer science are made usable to practicing biologists through open source platforms and how biologists can themselves participate in their further development.
Muscular hydrostats (such as mollusks), and fluid-filled animals (such as annelids), can exploit their constant-volume tissues to transfer forces and displacements in predictable ways, much as articulated animals use hinges and levers. Although larval insects contain pressurized fluids, they also have internal air tubes that are compressible and, as a result, they have more uncontrolled degrees of freedom. Therefore, the mechanisms by which larval insects control their movements are expected to reveal useful strategies for designing soft biomimetic robots. Using caterpillars as a tractable model system, it is now possible to identify the biomechanical and neural strategies for controlling movements in such highly deformable animals. For example, the tobacco hornworm, Manduca sexta, can stiffen its body by increasing muscular tension (and therefore body pressure) but the internal cavity (hemocoel) is not iso-barometric, nor is pressure used to directly control the movements of its limbs. Instead, fluid and tissues flow within the hemocoel and the body is soft and flexible to conform to the substrate. Even the gut contributes to the biomechanics of locomotion; it is decoupled from the movements of the body wall and slides forward within the body cavity at the start of each step. During crawling the body is kept in tension for part of the stride and compressive forces are exerted on the substrate along the axis of the caterpillar, thereby using the environment as a skeleton. The timing of muscular activity suggests that crawling is coordinated by proleg-retractor motoneurons and that the large segmental muscles produce anterograde waves of lifting that do not require precise timing. This strategy produces a robust form of locomotion in which the kinematics changes little with orientation. In different species of caterpillar, the presence of prolegs on particular body segments is related to alternative kinematics such as "inching." This suggests a mechanism for the evolution of different gaits through changes in the usage of prolegs, rather than, through extensive alterations in the motor program controlling the body wall. Some of these findings are being used to design and test novel control-strategies for highly deformable robots. These "softworm" devices are providing new insights into the challenges faced by any soft animal navigating in a terrestrial environment.