Filter
Associated Lab
- Aso Lab (1) Apply Aso Lab filter
- Betzig Lab (1) Apply Betzig Lab filter
- Remove Clapham Lab filter Clapham Lab
- Harris Lab (1) Apply Harris Lab filter
- Hess Lab (1) Apply Hess Lab filter
- Lavis Lab (1) Apply Lavis Lab filter
- Lippincott-Schwartz Lab (1) Apply Lippincott-Schwartz Lab filter
- Rubin Lab (1) Apply Rubin Lab filter
- Saalfeld Lab (1) Apply Saalfeld Lab filter
Publication Date
Type of Publication
2 Publications
Showing 1-2 of 2 resultsOptical and electron microscopy have made tremendous inroads toward understanding the complexity of the brain. However, optical microscopy offers insufficient resolution to reveal subcellular details, and electron microscopy lacks the throughput and molecular contrast to visualize specific molecular constituents over millimeter-scale or larger dimensions. We combined expansion microscopy and lattice light-sheet microscopy to image the nanoscale spatial relationships between proteins across the thickness of the mouse cortex or the entire Drosophila brain. These included synaptic proteins at dendritic spines, myelination along axons, and presynaptic densities at dopaminergic neurons in every fly brain region. The technology should enable statistically rich, large-scale studies of neural development, sexual dimorphism, degree of stereotypy, and structural correlations to behavior or neural activity, all with molecular contrast.
Targeting small-molecule fluorescent indicators using genetically encoded protein tags yields new hybrid sensors for biological imaging. Optimization of such systems requires redesign of the synthetic indicator to allow cell-specific targeting without compromising the photophysical properties or cellular performance of the small-molecule probe. We developed a bright and sensitive Ca indicator by systematically exploring the relative configuration of dye and chelator, which can be targeted using the HaloTag self-labeling tag system. Our "isomeric tuning" approach is generalizable, yielding a far-red targetable indicator to visualize Ca fluxes in the primary cilium.