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    Stern Lab
    09/01/11 | Preparation of cuticles from unhatched first-instar Drosophila larvae.
    Stern DL, Sucena E
    Cold Spring Harb Protoc. 2011 Sep;2011(9):. doi: 10.1101/pdb.prot065532

    The finely sculpted cuticle of Drosophila carries a rich array of morphological details. Thus, cuticle examination has had a central role in the history of genetics. Studies of the Drosophila cuticle have focused mainly on first-instar larvae and adult cuticular morphology. This protocol describes the preparation of cuticles from larvae that have not yet hatched from the egg. It is designed for sampling all eggs laid by one or more females. This can be particularly useful, for example, when a mutation produces embryos that are unable to hatch from the egg.

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