custom | custom
Filter
facetapi-Q2b17qCsTdECvJIqZJgYMaGsr8vANl1n | block
Associated Lab
facetapi-PV5lg7xuz68EAY8eakJzrcmwtdGEnxR0 | block
Publication Date
facetapi-021SKYQnqXW6ODq5W5dPAFEDBaEJubhN | block
Type of Publication
general_search_page-panel_pane_1 | views_panes
1 Publications
Showing 1-1 of 1 resultsYour Criteria:
11/15/08 |
A highly sensitive fluorogenic probe for cytochrome P450 activity in live cells.
Bioorganic & Medicinal Chemistry Letters. 2008 Nov 15;18(22):5864-6. doi: 10.1016/j.bmcl.2008.06.015
A derivative of rhodamine 110 has been designed and assessed as a probe for cytochrome P450 activity. This probe is the first to utilize a ’trimethyl lock’ that is triggered by cleavage of an ether bond. In vitro, fluorescence was manifested by the CYP1A1 isozyme with k(cat)/K(M)=8.8x10(3)M(-1)s(-1) and K(M)=0.09microM. In cellulo, the probe revealed the induction of cytochrome P450 activity by the carcinogen 2,3,7,8-tetrachlorodibenzo-p-dioxin, and its repression by the chemoprotectant resveratrol.