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Svoboda LabMouseLight
03/12/19 |
Single-neuron axonal reconstruction: The search for a wiring diagram of the brain.
The Journal of Comparative Neurology. 2019 Mar 12:. doi: 10.1002/cne.24674
Reconstruction of the axonal projection patterns of single neurons has been an important tool for understanding both the diversity of cell types in the brain and the logic of information flow between brain regions. Innovative approaches now enable the complete reconstruction of axonal projection patterns of individual neurons with vastly increased throughput. Here we review how advances in genetic, imaging, and computational techniques have been exploited for axonal reconstruction. We also discuss how new innovations could enable the integration of genetic and physiological information with axonal morphology for producing a census of cell types in the mammalian brain at scale. This article is protected by copyright. All rights reserved.