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02/03/09 |
Imaging transcription in living cells.
Annual Review of Biophysics. 2009 Feb 3;38:173-96. doi: 10.1073/pnas.1100640108
The advent of new technologies for the imaging of living cells has made it possible to determine the properties of transcription, the kinetics of polymerase movement, the association of transcription factors, and the progression of the polymerase on the gene. We report here the current state of the field and the progress necessary to achieve a more complete understanding of the various steps in transcription. Our Consortium is dedicated to developing and implementing the technology to further this understanding.