jYCaMP: an optimized calcium indicator for two-photon imaging at fiber laser wavelengths.

janelia7_blocks-janelia7_biblio_header | block

Nature Methods. 2020 May 25;17(1):694-97. doi: 10.1038/s41592-020-0835-7

jYCaMP: an optimized calcium indicator for two-photon imaging at fiber laser wavelengths. Looger LabSchreiter LabPodgorski LabGENIE

Mohr MA, Bushey D, Aggarwal Abhi, Marvin JS, Kim JJ, Marquez EJ, Liang Y, Patel R, Macklin JJ, Lee C, Tsang A, Tsegaye G, Ahrens AM, Chen JL, Kim DS, Wong AM, Looger LL, Schreiter ER, Podgorski K

janelia7_blocks-janelia7_biblio_abstract | block

Abstract

Femtosecond lasers at fixed wavelengths above 1,000 nm are powerful, stable and inexpensive, making them promising sources for two-photon microscopy. Biosensors optimized for these wavelengths are needed for both next-generation microscopes and affordable turn-key systems. Here we report jYCaMP1, a yellow variant of the calcium indicator jGCaMP7 that outperforms its parent in mice and flies at excitation wavelengths above 1,000 nm and enables improved two-color calcium imaging with red fluorescent protein-based indicators.