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1 Janelia Publications

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    Ji LabGENIE
    08/17/14 | Multiplexed aberration measurement for deep tissue imaging in vivo.
    Wang C, Liu R, Milkie DE, Sun W, Tan Z, Kerlin A, Chen T, Kim DS, Ji N
    Nature Methods. 2014 Aug 17:. doi: 10.1038/nmeth.3068

    We describe an adaptive optics method that modulates the intensity or phase of light rays at multiple pupil segments in parallel to determine the sample-induced aberration. Applicable to fluorescent protein-labeled structures of arbitrary complexity, it allowed us to obtain diffraction-limited resolution in various samples in vivo. For the strongly scattering mouse brain, a single aberration correction improved structural and functional imaging of fine neuronal processes over a large imaging volume.

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