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1 Janelia Publications

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    Cui Lab
    12/22/14 | In vivo fluorescence microscopy via iterative multi-photon adaptive compensation technique.
    Kong L, Cui M
    Optical Express. 2014 Oct 6;22(20):23786-94. doi: 10.1364/OE.22.023786

    Iterative multi-photon adaptive compensation technique (IMPACT) has been developed for wavefront measurement and compensation in highly scattering tissues. Our previous report was largely based on the measurements of fixed tissue. Here we demonstrate the advantages of IMPACT for in vivo imaging and report the latest results. In particular, we show that IMPACT can be used for functional imaging of awake mice, and greatly improve the in vivo neuron imaging in mouse cortex at large depth (~660 microns). Moreover, IMPACT enables neuron imaging through the intact skull of adult mice, which promises noninvasive optical measurements in mouse brain.

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