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    Lavis LabTool Translation Team (T3) Project Technical Resources
    05/12/24 | Coumarin as a general switching auxiliary to prepare photochromic and spontaneously blinking fluorophores
    Jradi FM, English BP, Brown TA, Aaron J, Khuon S, Galbraith JA, Galbraith CG, Lavis LD
    bioRxiv. 2024 May 12:. doi: 10.1101/2024.05.12.593749

    Single-molecule localization microscopy (SMLM) uses activatable or switchable fluorophores to create non-diffraction limited maps of molecular location in biological samples. Despite the utility of this imaging technique, the portfolio of appropriate labels for SMLM remains limited. Here, we describe a general strategy for the construction of “glitter bomb” labels by simply combining rhodamine and coumarin dyes though an amide bond. Condensation of the ortho-carboxyl group on the pendant phenyl ring of rhodamine dyes with a 7-aminocoumarin yields photochromic or spontaneously blinking fluorophores depending on the parent rhodamine structure. We apply this strategy to prepare labels useful super-resolution experiments in fixed cells using different attachment techniques. This general glitter bomb strategy should lead to improved labels for SMLM, ultimately enabling the creation of detailed molecular maps in biological samples.

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