filters_region_cap | custom
Filter
facetapi-Q2b17qCsTdECvJIqZJgYMaGsr8vANl1n | block
Associated Lab
facetapi-PV5lg7xuz68EAY8eakJzrcmwtdGEnxR0 | block
Publication Date
- Remove April 15, 2018 filter April 15, 2018
- Remove April 2018 filter April 2018
- Remove 2018 filter 2018
general_search_page-panel_pane_1 | views_panes
1 Janelia Publications
Showing 1-1 of 1 resultsYour Criteria:
Ji Lab
04/15/18 |
Three-photon fluorescence microscopy with an axially elongated Bessel focus.
Optics Letters. 2018 Apr 15;43(8):1914-1917. doi: 10.1364/OL.43.001914
Volumetric imaging tools that are simple to adopt, flexible, and robust are in high demand in the field of neuroscience, where the ability to image neurons and their networks with high spatiotemporal resolution is essential. Using an axially elongated focus approximating a Bessel beam, in combination with two-photon fluorescence microscopy, has proven successful at such an endeavor. Here, we demonstrate three-photon fluorescence imaging with an axially extended Bessel focus. We use an axicon-based module that allowed for the generation of Bessel foci of varying numerical apertures and axial lengths, and apply this volumetric imaging tool to image mouse brain slices and for in vivo imaging of the mouse brain.