Main Menu (Mobile)- Block

Main Menu - Block

custom | custom

Search Results

filters_region_cap | custom

Filter

facetapi-Q2b17qCsTdECvJIqZJgYMaGsr8vANl1n | block

Associated Lab

facetapi-PV5lg7xuz68EAY8eakJzrcmwtdGEnxR0 | block

Publication Date

general_search_page-panel_pane_1 | views_panes

2 Janelia Publications

Showing 1-2 of 2 results
Your Criteria:
    Lippincott-Schwartz Lab
    12/30/16 | Live cell single molecule-guided Bayesian localization super resolution microscopy.
    Xu F, Zhang M, He W, Han R, Xue F, Liu Z, Zhang F, Lippincott-Schwartz J, Xu P
    Cell Research. 2016 Dec 30:. doi: 10.1038/cr.2015.160
    Lippincott-Schwartz Lab
    12/14/16 | The nanoscale spatial organization of B cell receptors on IgM- and IgG-expressing human B cells.
    Lee J, Sengupta P, Brzostowski J, Lippincott-Schwartz J, Pierce SK
    Molecular Biology of the Cell. 2016 Dec 14;28(4):511-23. doi: 10.1091/mbc.E16-06-0452

    B cell activation is initiated by the binding of antigen to the B cell receptor (BCR). Here we used dSTORM super resolution imaging to characterize the nanoscale spatial organization of IgM and IgG BCRs on the surfaces of resting and antigen-activated human peripheral blood B cells. We provide insights into both the fundamental process of antigen-driven BCR clustering as well as differences in the spatial organization of IgM and IgG BCRs that may contribute to the characteristic differences in the responses of naïve and memory B cells to antigen. We provide evidence that although both IgM and IgG BCRs reside in highly heterogeneous protein islands that vary in both size and number of BCR single molecule localizations, both resting and activated B cells intrinsically maintain a high frequency of single isolated BCR localizations, which likely represent BCR monomers. IgG BCRs are more clustered than IgM BCRs on resting cells and form larger protein islands following antigen activation. Small dense BCR clusters likely formed via protein-protein interactions are present on the surface of resting cells and antigen activation induces these to come together to form less dense, larger islands, a process likely governed, at least in part, by protein-lipid interactions.

    View Publication Page