Filter
Associated Lab
- Ahrens Lab (4) Apply Ahrens Lab filter
- Aso Lab (6) Apply Aso Lab filter
- Betzig Lab (4) Apply Betzig Lab filter
- Branson Lab (2) Apply Branson Lab filter
- Card Lab (6) Apply Card Lab filter
- Clapham Lab (1) Apply Clapham Lab filter
- Darshan Lab (4) Apply Darshan Lab filter
- Dickson Lab (4) Apply Dickson Lab filter
- Dudman Lab (2) Apply Dudman Lab filter
- Espinosa Medina Lab (1) Apply Espinosa Medina Lab filter
- Feliciano Lab (1) Apply Feliciano Lab filter
- Fitzgerald Lab (5) Apply Fitzgerald Lab filter
- Funke Lab (12) Apply Funke Lab filter
- Harris Lab (7) Apply Harris Lab filter
- Hermundstad Lab (2) Apply Hermundstad Lab filter
- Hess Lab (6) Apply Hess Lab filter
- Jayaraman Lab (1) Apply Jayaraman Lab filter
- Karpova Lab (2) Apply Karpova Lab filter
- Keller Lab (1) Apply Keller Lab filter
- Lavis Lab (10) Apply Lavis Lab filter
- Lee (Albert) Lab (5) Apply Lee (Albert) Lab filter
- Li Lab (1) Apply Li Lab filter
- Lippincott-Schwartz Lab (9) Apply Lippincott-Schwartz Lab filter
- Liu (Zhe) Lab (3) Apply Liu (Zhe) Lab filter
- Looger Lab (5) Apply Looger Lab filter
- Pachitariu Lab (5) Apply Pachitariu Lab filter
- Podgorski Lab (2) Apply Podgorski Lab filter
- Reiser Lab (6) Apply Reiser Lab filter
- Romani Lab (3) Apply Romani Lab filter
- Rubin Lab (6) Apply Rubin Lab filter
- Saalfeld Lab (6) Apply Saalfeld Lab filter
- Scheffer Lab (2) Apply Scheffer Lab filter
- Schreiter Lab (6) Apply Schreiter Lab filter
- Shroff Lab (1) Apply Shroff Lab filter
- Spruston Lab (3) Apply Spruston Lab filter
- Stern Lab (4) Apply Stern Lab filter
- Sternson Lab (2) Apply Sternson Lab filter
- Stringer Lab (5) Apply Stringer Lab filter
- Svoboda Lab (5) Apply Svoboda Lab filter
- Tebo Lab (2) Apply Tebo Lab filter
- Tervo Lab (1) Apply Tervo Lab filter
- Tillberg Lab (3) Apply Tillberg Lab filter
- Truman Lab (2) Apply Truman Lab filter
- Turaga Lab (2) Apply Turaga Lab filter
- Turner Lab (11) Apply Turner Lab filter
- Vale Lab (1) Apply Vale Lab filter
- Wang (Meng) Lab (3) Apply Wang (Meng) Lab filter
- Wang (Shaohe) Lab (1) Apply Wang (Shaohe) Lab filter
Associated Project Team
- CellMap (1) Apply CellMap filter
- COSEM (1) Apply COSEM filter
- Fly Descending Interneuron (2) Apply Fly Descending Interneuron filter
- Fly Functional Connectome (2) Apply Fly Functional Connectome filter
- Fly Olympiad (1) Apply Fly Olympiad filter
- FlyEM (4) Apply FlyEM filter
- FlyLight (12) Apply FlyLight filter
- GENIE (5) Apply GENIE filter
- MouseLight (1) Apply MouseLight filter
- Tool Translation Team (T3) (3) Apply Tool Translation Team (T3) filter
Associated Support Team
- Anatomy and Histology (2) Apply Anatomy and Histology filter
- Cryo-Electron Microscopy (3) Apply Cryo-Electron Microscopy filter
- Fly Facility (10) Apply Fly Facility filter
- Gene Targeting and Transgenics (2) Apply Gene Targeting and Transgenics filter
- Integrative Imaging (1) Apply Integrative Imaging filter
- Janelia Experimental Technology (4) Apply Janelia Experimental Technology filter
- Molecular Genomics (1) Apply Molecular Genomics filter
- Primary & iPS Cell Culture (1) Apply Primary & iPS Cell Culture filter
- Project Technical Resources (9) Apply Project Technical Resources filter
- Scientific Computing Software (3) Apply Scientific Computing Software filter
- Scientific Computing Systems (2) Apply Scientific Computing Systems filter
- Viral Tools (2) Apply Viral Tools filter
Publication Date
- December 2023 (9) Apply December 2023 filter
- November 2023 (17) Apply November 2023 filter
- October 2023 (14) Apply October 2023 filter
- September 2023 (15) Apply September 2023 filter
- August 2023 (18) Apply August 2023 filter
- July 2023 (11) Apply July 2023 filter
- June 2023 (24) Apply June 2023 filter
- May 2023 (15) Apply May 2023 filter
- April 2023 (12) Apply April 2023 filter
- March 2023 (15) Apply March 2023 filter
- February 2023 (12) Apply February 2023 filter
- January 2023 (13) Apply January 2023 filter
- Remove 2023 filter 2023
175 Janelia Publications
Showing 171-175 of 175 resultsVisceral sensory pathways mediate homeostatic reflexes, the dysfunction of which leads to many neurological disorders. The Bezold-Jarisch reflex (BJR), first described in 1867, is a cardioinhibitory reflex that is speculated to be mediated by vagal sensory neurons (VSNs) that also triggers syncope. However, the molecular identity, anatomical organization, physiological characteristics and behavioural influence of cardiac VSNs remain mostly unknown. Here we leveraged single-cell RNA-sequencing data and HYBRiD tissue clearing to show that VSNs that express neuropeptide Y receptor Y2 (NPY2R) predominately connect the heart ventricular wall to the area postrema. Optogenetic activation of NPY2R VSNs elicits the classic triad of BJR responses-hypotension, bradycardia and suppressed respiration-and causes an animal to faint. Photostimulation during high-resolution echocardiography and laser Doppler flowmetry with behavioural observation revealed a range of phenotypes reflected in clinical syncope, including reduced cardiac output, cerebral hypoperfusion, pupil dilation and eye-roll. Large-scale Neuropixels brain recordings and machine-learning-based modelling showed that this manipulation causes the suppression of activity across a large distributed neuronal population that is not explained by changes in spontaneous behavioural movements. Additionally, bidirectional manipulation of the periventricular zone had a push-pull effect, with inhibition leading to longer syncope periods and activation inducing arousal. Finally, ablating NPY2R VSNs specifically abolished the BJR. Combined, these results demonstrate a genetically defined cardiac reflex that recapitulates characteristics of human syncope at physiological, behavioural and neural network levels.
The hippocampus is critical for recollecting and imagining experiences. This is believed to involve voluntarily drawing from hippocampal memory representations of people, events, and places, including maplike representations of familiar environments. However, whether representations in such "cognitive maps" can be volitionally accessed is unknown. We developed a brain-machine interface to test whether rats can do so by controlling their hippocampal activity in a flexible, goal-directed, and model-based manner. We found that rats can efficiently navigate or direct objects to arbitrary goal locations within a virtual reality arena solely by activating and sustaining appropriate hippocampal representations of remote places. This provides insight into the mechanisms underlying episodic memory recall, mental simulation and planning, and imagination and opens up possibilities for high-level neural prosthetics that use hippocampal representations.
Neurons integrate synaptic inputs within their dendrites and produce spiking outputs, which then propagate down the axon and back into the dendrites where they contribute to plasticity. Mapping the voltage dynamics in dendritic arbors of live animals is crucial for understanding neuronal computation and plasticity rules. Here we combine patterned channelrhodopsin activation with dual-plane structured illumination voltage imaging, for simultaneous perturbation and monitoring of dendritic and somatic voltage in Layer 2/3 pyramidal neurons in anesthetized and awake mice. We examined the integration of synaptic inputs and compared the dynamics of optogenetically evoked, spontaneous, and sensory-evoked back-propagating action potentials (bAPs). Our measurements revealed a broadly shared membrane voltage throughout the dendritic arbor, and few signatures of electrical compartmentalization among synaptic inputs. However, we observed spike rate acceleration-dependent propagation of bAPs into distal dendrites. We propose that this dendritic filtering of bAPs may play a critical role in activity-dependent plasticity.
Life exists in three dimensions, but until the turn of the century most electron microscopy methods provided only 2D image data. Recently, electron microscopy techniques capable of delving deep into the structure of cells and tissues have emerged, collectively called volume electron microscopy (vEM). Developments in vEM have been dubbed a quiet revolution as the field evolved from established transmission and scanning electron microscopy techniques, so early publications largely focused on the bioscience applications rather than the underlying technological breakthroughs. However, with an explosion in the uptake of vEM across the biosciences and fast-paced advances in volume, resolution, throughput and ease of use, it is timely to introduce the field to new audiences. In this Primer, we introduce the different vEM imaging modalities, the specialized sample processing and image analysis pipelines that accompany each modality and the types of information revealed in the data. We showcase key applications in the biosciences where vEM has helped make breakthrough discoveries and consider limitations and future directions. We aim to show new users how vEM can support discovery science in their own research fields and inspire broader uptake of the technology, finally allowing its full adoption into mainstream biological imaging.
Mechanisms that entrain and drive rhythmic epileptiform discharges remain debated. Traditionally, this quest has been focusing on interneuronal networks driven by GABAergic connections that activate synaptic or extrasynaptic receptors. However, synchronised interneuronal discharges could also trigger a transient elevation of extracellular GABA across the tissue volume, thus raising tonic GABAA receptor conductance (Gtonic) in multiple cells. Here, we use patch-clamp GABA ‘sniffer’ and optical GABA sensor to show that periodic epileptiform discharges are preceded by region-wide, rising waves of extracellular GABA. Neural network simulations that incorporate volume-transmitted GABA signals point to mechanistic principles underpinning this relationship. We validate this hypothesis using simultaneous patch-clamp recordings from multiple nerve cells, selective optogenetic stimulation of fast-spiking interneurons. Critically, we manipulate GABA uptake to suppress extracellular GABA waves but not synaptic GABAergic transmission, which shows a clear effect on rhythm generation. Our findings thus unveil a key role of extrasynaptic, volume-transmitted GABA actions in pacing regenerative rhythmic activity in brain networks.