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1 Janelia Publications

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    Gonen Lab
    04/04/14 | Protein structure determination by MicroED.
    Nannenga BL, Gonen T
    Current Opinion in Structural Biology. 2014 Apr 4;27C:24-31. doi: 10.1016/j.sbi.2014.03.004

    In this review we discuss the current advances relating to structure determination from protein microcrystals with special emphasis on the newly developed method called MicroED. This method uses a transmission electron cryo-microscope to collect electron diffraction data from extremely small 3-dimensional (3D) crystals. MicroED has been used to solve the 3D structure of the model protein lysozyme to 2.9A resolution. As the method further matures, MicroED promises to offer a unique and widely applicable approach to protein crystallography using nanocrystals.

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