Filter
Associated Lab
- Aguilera Castrejon Lab (1) Apply Aguilera Castrejon Lab filter
- Ahrens Lab (4) Apply Ahrens Lab filter
- Aso Lab (4) Apply Aso Lab filter
- Betzig Lab (6) Apply Betzig Lab filter
- Beyene Lab (2) Apply Beyene Lab filter
- Bock Lab (3) Apply Bock Lab filter
- Branson Lab (5) Apply Branson Lab filter
- Card Lab (4) Apply Card Lab filter
- Cardona Lab (6) Apply Cardona Lab filter
- Clapham Lab (5) Apply Clapham Lab filter
- Darshan Lab (1) Apply Darshan Lab filter
- Dickson Lab (2) Apply Dickson Lab filter
- Druckmann Lab (4) Apply Druckmann Lab filter
- Dudman Lab (3) Apply Dudman Lab filter
- Feliciano Lab (1) Apply Feliciano Lab filter
- Fetter Lab (4) Apply Fetter Lab filter
- Fitzgerald Lab (2) Apply Fitzgerald Lab filter
- Freeman Lab (1) Apply Freeman Lab filter
- Funke Lab (4) Apply Funke Lab filter
- Gonen Lab (9) Apply Gonen Lab filter
- Grigorieff Lab (6) Apply Grigorieff Lab filter
- Harris Lab (5) Apply Harris Lab filter
- Heberlein Lab (3) Apply Heberlein Lab filter
- Hermundstad Lab (1) Apply Hermundstad Lab filter
- Hess Lab (3) Apply Hess Lab filter
- Jayaraman Lab (3) Apply Jayaraman Lab filter
- Ji Lab (5) Apply Ji Lab filter
- Johnson Lab (1) Apply Johnson Lab filter
- Karpova Lab (2) Apply Karpova Lab filter
- Keleman Lab (1) Apply Keleman Lab filter
- Keller Lab (6) Apply Keller Lab filter
- Koay Lab (5) Apply Koay Lab filter
- Lavis Lab (12) Apply Lavis Lab filter
- Lee (Albert) Lab (2) Apply Lee (Albert) Lab filter
- Li Lab (3) Apply Li Lab filter
- Lippincott-Schwartz Lab (11) Apply Lippincott-Schwartz Lab filter
- Liu (Zhe) Lab (3) Apply Liu (Zhe) Lab filter
- Looger Lab (8) Apply Looger Lab filter
- Magee Lab (1) Apply Magee Lab filter
- Menon Lab (1) Apply Menon Lab filter
- Murphy Lab (1) Apply Murphy Lab filter
- O'Shea Lab (1) Apply O'Shea Lab filter
- Pachitariu Lab (2) Apply Pachitariu Lab filter
- Pavlopoulos Lab (2) Apply Pavlopoulos Lab filter
- Pedram Lab (1) Apply Pedram Lab filter
- Podgorski Lab (2) Apply Podgorski Lab filter
- Reiser Lab (4) Apply Reiser Lab filter
- Riddiford Lab (1) Apply Riddiford Lab filter
- Romani Lab (3) Apply Romani Lab filter
- Rubin Lab (7) Apply Rubin Lab filter
- Saalfeld Lab (5) Apply Saalfeld Lab filter
- Scheffer Lab (4) Apply Scheffer Lab filter
- Schreiter Lab (4) Apply Schreiter Lab filter
- Singer Lab (5) Apply Singer Lab filter
- Spruston Lab (8) Apply Spruston Lab filter
- Stern Lab (6) Apply Stern Lab filter
- Stringer Lab (1) Apply Stringer Lab filter
- Svoboda Lab (11) Apply Svoboda Lab filter
- Tebo Lab (6) Apply Tebo Lab filter
- Tervo Lab (2) Apply Tervo Lab filter
- Tillberg Lab (1) Apply Tillberg Lab filter
- Truman Lab (8) Apply Truman Lab filter
- Turaga Lab (7) Apply Turaga Lab filter
- Wang (Shaohe) Lab (2) Apply Wang (Shaohe) Lab filter
- Zlatic Lab (5) Apply Zlatic Lab filter
Associated Project Team
Publication Date
- December 2018 (14) Apply December 2018 filter
- November 2018 (24) Apply November 2018 filter
- October 2018 (27) Apply October 2018 filter
- September 2018 (15) Apply September 2018 filter
- August 2018 (28) Apply August 2018 filter
- July 2018 (15) Apply July 2018 filter
- June 2018 (23) Apply June 2018 filter
- May 2018 (17) Apply May 2018 filter
- April 2018 (23) Apply April 2018 filter
- March 2018 (20) Apply March 2018 filter
- February 2018 (13) Apply February 2018 filter
- January 2018 (13) Apply January 2018 filter
- Remove 2018 filter 2018
Type of Publication
232 Publications
Showing 231-232 of 232 resultsThe hexameric AAA ATPase VPS4 facilitates ESCRT III filament disassembly on diverse intracellular membranes. ESCRT III components and VPS4 have been localized to the ciliary transition zone and spindle poles and reported to affect centrosome duplication and spindle pole stability. How the canonical ESCRT pathway could mediate these events is unclear. We studied the association of VPS4 with centrosomes and found that GFP-VPS4 was a dynamic component of both mother and daughter centrioles. A mutant, VPS4, which can't hydrolyze ATP, was less dynamic and accumulated at centrosomes. Centrosome localization of the VPS4mutant, caused reduced γ-tubulin levels at centrosomes and consequently decreased microtubule growth and altered centrosome positioning. In addition, preventing VPS4 ATP hydrolysis nearly eliminated centriolar satellites and paused ciliogensis after formation of the ciliary vesicle. Zebrafish embryos injected with GFP-VPS4mRNA were less viable, exhibited developmental defects and had fewer cilia in Kupffer's vesicle. Surprisingly, ESCRT III proteins seldom localized to centrosomes and their depletion did not lead to these phenotypes. Our data support an ESCRT III-independent function for VPS4 at the centrosome and reveal that this evolutionary conserved AAA ATPase influences diverse centrosome functions and, as a result, global cellular architecture and development.
We demonstrate STED microscopy of whole bacterial and eukaryotic cells using fluorogenic labels that reversibly bind to their target structure. A constant exchange of labels guarantees the removal of photobleached fluorophores and their replacement by intact fluorophores, thereby circumventing bleaching-related limitations of STED super-resolution imaging. We achieve a constant labeling density and demonstrate a fluorescence signal for long and theoretically unlimited acquisition times. Using this concept, we demonstrate whole-cell, 3D, multi-color and live cell STED microscopy.