Filter
Associated Lab
- Betzig Lab (4) Apply Betzig Lab filter
- Fetter Lab (1) Apply Fetter Lab filter
- Gonen Lab (1) Apply Gonen Lab filter
- Harris Lab (1) Apply Harris Lab filter
- Lavis Lab (5) Apply Lavis Lab filter
- Liu (Zhe) Lab (8) Apply Liu (Zhe) Lab filter
- Rubin Lab (1) Apply Rubin Lab filter
- Singer Lab (5) Apply Singer Lab filter
- Remove Tjian Lab filter Tjian Lab
- Wu Lab (1) Apply Wu Lab filter
Associated Project Team
Publication Date
- 2016 (3) Apply 2016 filter
- 2015 (6) Apply 2015 filter
- 2014 (4) Apply 2014 filter
- 2013 (1) Apply 2013 filter
- 2012 (1) Apply 2012 filter
- 2011 (4) Apply 2011 filter
- 2010 (2) Apply 2010 filter
- 2009 (4) Apply 2009 filter
- 2008 (7) Apply 2008 filter
- 2007 (5) Apply 2007 filter
- 2006 (6) Apply 2006 filter
- 2005 (4) Apply 2005 filter
- 2004 (5) Apply 2004 filter
- 2003 (6) Apply 2003 filter
- 2002 (5) Apply 2002 filter
- 1994 (1) Apply 1994 filter
Type of Publication
64 Publications
Showing 61-64 of 64 resultsThe insulin signaling pathway, which is conserved in evolution from flies to humans, evolved to allow a fast response to changes in nutrient availability while keeping glucose concentration constant in serum. Here we show that, both in Drosophila and mammals, insulin receptor (InR) represses its own synthesis by a feedback mechanism directed by the transcription factor dFOXO/FOXO1. In Drosophila, dFOXO is responsible for activating transcription of dInR, and nutritional conditions can modulate this effect. Starvation up-regulates mRNA of dInR in wild-type but not dFOXO-deficient flies. Importantly, FOXO1 acts in mammalian cells like its Drosophila counterpart, up-regulating the InR mRNA level upon fasting. Mammalian cells up-regulate the InR mRNA in the absence of serum, conditions that induce the dephosphorylation and activation of FOXO1. Interestingly, insulin is able to reverse this effect. Therefore, dFOXO/FOXO1 acts as an insulin sensor to activate insulin signaling, allowing a fast response to the hormone after each meal. Our results reveal a key feedback control mechanism for dFOXO/FOXO1 in regulating metabolism and insulin signaling.
Drosophila TATA-box-binding protein (TBP)-related factor 2 (TRF2) is a member of a family of TBP-related factors present in metazoan organisms. Recent evidence suggests that TRF2s are required for proper embryonic development and differentiation. However, true target promoters and the mechanisms by which TRF2 operates to control transcription remain elusive. Here we report the antibody affinity purification of a Drosophila TRF2-containing complex that contains components of the nucleosome remodelling factor (NURF) chromatin remodelling complex as well as the DNA replication-related element (DRE)-binding factor DREF. This latter finding led us to potential target genes containing TRF2-responsive promoters. We have used a combination of in vitro and in vivo assays to show that the DREF-containing TRF2 complex directs core promoter recognition of the proliferating cell nuclear antigen (PCNA) gene. We also identified additional TRF2-responsive target genes involved in DNA replication and cell proliferation. These data suggest that TRF2 functions as a core promoter-selectivity factor responsible for coordinating transcription of a subset of genes in Drosophila.
The eukaryotic core promoter recognition complex was generally thought to play an essential but passive role in the regulation of gene expression. However, recent evidence now indicates that core promoter recognition complexes together with ’non-prototypical’ subunits may have a vital regulatory function in driving cell-specific programmes of transcription during development. Furthermore, new roles for components of these complexes have been identified beyond development; for example, in mediating interactions with chromatin and in maintaining active gene expression across cell divisions.
Understanding the diverse activities of the multisubunit core promoter recognition complex TFIID in vivo requires knowledge of how individual subunits contribute to overall functions of this TATA box-binding protein (TBP)/TBP-associated factor (TAF) complex. By generating altered holo-TFIID complexes in Drosophila we identify the ETO domain of TAF4 as a coactivator domain likely targeted by Pygopus, a protein that is required for Wingless-induced transcription of naked cuticle. These results establish a coactivator function of TAF4 and provide a strategy to dissect mechanisms of TFIID function in vivo.