Filter
Associated Lab
- Ahrens Lab (1) Apply Ahrens Lab filter
- Betzig Lab (8) Apply Betzig Lab filter
- Clapham Lab (2) Apply Clapham Lab filter
- Dudman Lab (2) Apply Dudman Lab filter
- Harris Lab (3) Apply Harris Lab filter
- Hess Lab (2) Apply Hess Lab filter
- Ji Lab (1) Apply Ji Lab filter
- Keller Lab (2) Apply Keller Lab filter
- Lavis Lab (132) Apply Lavis Lab filter
- Lippincott-Schwartz Lab (6) Apply Lippincott-Schwartz Lab filter
- Liu (Zhe) Lab (15) Apply Liu (Zhe) Lab filter
- Looger Lab (8) Apply Looger Lab filter
- Podgorski Lab (2) Apply Podgorski Lab filter
- Schreiter Lab (5) Apply Schreiter Lab filter
- Singer Lab (6) Apply Singer Lab filter
- Spruston Lab (1) Apply Spruston Lab filter
- Stern Lab (2) Apply Stern Lab filter
- Sternson Lab (1) Apply Sternson Lab filter
- Svoboda Lab (4) Apply Svoboda Lab filter
- Tebo Lab (1) Apply Tebo Lab filter
- Tillberg Lab (1) Apply Tillberg Lab filter
- Tjian Lab (5) Apply Tjian Lab filter
- Turner Lab (2) Apply Turner Lab filter
Associated Project Team
Publication Date
- 2024 (5) Apply 2024 filter
- 2023 (11) Apply 2023 filter
- 2022 (13) Apply 2022 filter
- 2021 (10) Apply 2021 filter
- 2020 (9) Apply 2020 filter
- 2019 (6) Apply 2019 filter
- 2018 (12) Apply 2018 filter
- 2017 (16) Apply 2017 filter
- 2016 (13) Apply 2016 filter
- 2015 (5) Apply 2015 filter
- 2014 (7) Apply 2014 filter
- 2013 (4) Apply 2013 filter
- 2012 (4) Apply 2012 filter
- 2011 (5) Apply 2011 filter
- 2010 (1) Apply 2010 filter
- 2009 (2) Apply 2009 filter
- 2008 (4) Apply 2008 filter
- 2007 (3) Apply 2007 filter
- 2006 (2) Apply 2006 filter
Type of Publication
132 Publications
Showing 131-132 of 132 resultsTraditional small-molecule fluorophores are always fluorescent. This attribute can obscure valuable information in biological experiments. Here, we report on a versatile "latent" fluorophore that overcomes this limitation. At the core of the latent fluorophore is a derivative of rhodamine in which one nitrogen is modified as a urea. That modification enables rhodamine to retain half of its fluorescence while facilitating conjugation to a target molecule. The other nitrogen of rhodamine is modified with a "trimethyl lock", which enables fluorescence to be unmasked fully by a single user-designated chemical reaction. An esterase-reactive latent fluorophore was synthesized in high yield and attached covalently to a cationic protein. The resulting conjugate was not fluorescent in the absence of esterases. The enzymatic activity of esterases in endocytic vesicles and the cytosol educed fluorescence, enabling the time-lapse imaging of endocytosis into live human cells and thus providing unprecedented spatiotemporal resolution of this process. The modular design of this "fluorogenic label" enables the facile synthesis of an ensemble of small-molecule probes for the illumination of numerous biochemical and cell biological processes.