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06/05/17 |
Quantitative mRNA imaging throughout the entire Drosophila brain.
Nature Methods. 2017 Jun 05;14(7):703-6. doi: 10.1038/nmeth.4309
We describe a fluorescence in situ hybridization method that permits detection of the localization and abundance of single mRNAs (smFISH) in cleared whole-mount adult Drosophila brains. The approach is rapid and multiplexable and does not require molecular amplification; it allows facile quantification of mRNA expression with subcellular resolution on a standard confocal microscope. We further demonstrate single-mRNA detection across the entire brain using a custom Bessel beam structured illumination microscope (BB-SIM).