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1 Janelia Publications

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    Lavis Lab
    12/05/23 | Imaging neuronal voltage beyond the scattering limit
    Tsai-Wen Chen , Xian-Bin Huang , Sarah E. Plutkis , Katie L. Holland , Luke D. Lavis , Bei-Jung Lin
    bioRxiv. 2023 Dec 05:. doi: 10.1101/2023.12.03.568403

    Voltage imaging is a promising technique for high-speed recording of neuronal population activity. However, tissue scattering severely limits its application in dense neuronal populations. Here, we adopted the principle of localization microscopy, a technique that enables super-resolution imaging of single-molecules, to resolve dense neuronal activities in vivo. Leveraging the sparse activation of neurons during action potentials (APs), we precisely localize the fluorescence change associated with each AP, creating a super-resolution image of neuronal activities. This approach, termed Activity Localization Imaging (ALI), identifies overlapping neurons and separates their activities with over 10-fold greater precision than what tissue scattering permits. Using ALI, we simultaneously recorded over a hundred densely-labeled CA1 neurons, creating a map of hippocampal theta oscillation at single-cell and single-cycle resolution.

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