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2 Janelia Publications
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11/18/11 |
Facile and general synthesis of photoactivatable xanthene dyes.
Angewandte Chemie. 2011 Nov 18;50:11206-9. doi: 10.1002/anie.201104571
Despite the apparent simplicity of the xanthene fluorophores, the preparation of caged derivatives with free carboxy groups remains a synthetic challenge. A straightforward and flexible strategy for preparing rhodamine and fluorescein derivatives was developed using reduced, “leuco” intermediates.
Betzig LabJi Lab
11/01/11 |
Pupil-segmentation-based adaptive optical microscopy with full-pupil illumination.
Optics Letters. 2011 Nov 1;36(21):4206-8. doi: 10.1364/OL.36.004206
Optical aberrations deteriorate the performance of microscopes. Adaptive optics can be used to improve imaging performance via wavefront shaping. Here, we demonstrate a pupil-segmentation based adaptive optical approach with full-pupil illumination. When implemented in a two-photon fluorescence microscope, it recovers diffraction-limited performance and improves imaging signal and resolution.