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Services
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The facility serves HHMI labs and their collaborators. We provide services spanning the entire cryo-EM workflow.
High-quality, high-throughput data collection on a Krios microscope
We do high quality high throughput data collection for both single particle and tomography projects on the Krios microscopes. Facility staff operate the microscope, optimize imaging conditions/parameters, and set up automated data collection while users focus on evaluating the sample and selecting targets for data collection (remotely or in-person) on cryo-EM grids provided by users that are ready for imaging.
CryoFIB milling
For cells frozen on a cryo-EM grid that are too thick to image on the Krios microscope directly, we offer an FEI Aquilos Cryo Focused Ion Beam (cryoFIB) system to mill regions of interest into a thin lamella. The cell lamella can then be loaded into the Krios microscope for imaging or tomography data collection.
To help locate regions of interest before cryoFIB milling, a Leica cryoCLEM light microscope is available to image the cryo-EM grid.
Additional services
- Preparation of samples not prepared on a cryo-EM grid (e.g., protein complex solution or purified cellular components or cells), including:
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Calibrating and evaluating samples by negative stain EM
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Plunge freezing
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Utilizing the Chameleon or cryoFIB milling systems, if needed
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Screening on a cryo TEM
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- Image processing
- 3D reconstruction
Facility staff will interact closely with lab members during these processes. This type of work is research-oriented by nature and should be considered as a collaboration between the lab and the facility.
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Quick Links
Our services are for HHMI labs and their collaborators.
Review policies and instructions before reserving time on equipment or sending any shipments.
Reserve time on our equipment.
Complete the Grid Submission Form before shipping cryo-EM grids for data collection.
Instructions for shipping cryo-EM grids.
Instructions for shipping solution samples.