Microscopy

The correct positioning of optical elements like lenses, mirrors, and AOTFs in optical systems can often be tedious. However, precise and reproducible alignment tools can simplify the positioning of optical elements tremendously.

The standard reflection objective is designed to work in air with reasonably high resolution. It is a lightweight, simple structure (only one or two mirrors are needed) with little chromatic dispersion and a relatively long working distance. However, it cannot be used in an immersion medium other than air. Otherwise, the light passing through the objective will suffer from considerable aberrations, and those will reduce resolution at the sample.

To obtain the documentation, designs, and parts list, please contact innovation@janelia.hhmi.org.

See additional explanations from the scientists here: https://www.janelia.org/lab/keller-lab/microscopes.

This technology advances imaging techniques of biomicroscopy using an innovative type of a wide-field Multi-Focus microscope to enable fast, high-resolution 3D imaging.

The Lattice Light involves using a Bessel beam to illuminate a sample with light sheets that are sufficiently thin to achieve isotropic 3D resolution high-speed 3D fluorescent imaging. As a result, the technology can visualize cellular processes in spatiotemporal detail not attainable with other fluorescent microscopy techniques.

Repeatable microscope observations at high magnification and resolution are difficult when using current slide holders for oil immersion due to the objective, oil, and slide movement on the microscope stage. Therefore, a unique microscope slide holder was designed to hold slides securely for oil immersion microscopy and other microscope systems that require a fixed slide position.