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02/24/24 |
A series of spontaneously blinking dyes for super-resolution microscopy
bioRxiv. 2024 Feb 24:. doi: 10.1101/2024.02.23.581625
Spontaneously blinking fluorophores permit the detection and localization of individual molecules without reducing buffers or caging groups, thus simplifying single-molecule localization microscopy (SMLM). The intrinsic blinking properties of such dyes are dictated by molecular structure and modulated by environment, which can limit utility. We report a series of tuned spontaneously blinking dyes with duty cycles that span two orders of magnitude, allowing facile SMLM in cells and dense biomolecular structures.