Filter
Associated Lab
- Remove Lavis Lab filter Lavis Lab
Publication Date
- Remove 2010-12-31 19:00 ā 2011-12-31 19:00 filter 2010-12-31 19:00 ā 2011-12-31 19:00
- December 16, 2011 (1) Apply December 16, 2011 filter
- December 1, 2011 (1) Apply December 1, 2011 filter
- Remove December 2011 filter December 2011
Type of Publication
2 Publications
Showing 1-2 of 2 resultsSmall molecule fluorophores are essential tools for chemical biology. A benefit of synthetic dyes is the ability to employ chemical approaches to control the properties and direct the position of the fluorophore. Applying modern synthetic organic chemistry strategies enables efficient tailoring of the chemical structure to obtain probes for specific biological experiments. Chemistry can also be used to activate fluorophores; new fluorogenic enzyme substrates and photoactivatable compounds with improved properties have been prepared that facilitate advanced imaging experiments with low background fluorescence. Finally, chemical reactions in live cells can be used to direct the spatial distribution of the fluorophore, allowing labeling of defined cellular regions with synthetic dyes.
A unified, convenient, and efficient strategy for the preparation of rhodamines and N,Nā-diacylated rhodamines has been developed. Fluorescein ditriflates were found to undergo palladium-catalyzed C-N cross-coupling with amines, amides, carbamates, and other nitrogen nucleophiles to provide direct access to known and novel rhodamine derivatives, including fluorescent dyes, quenchers, and latent fluorophores.