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05/25/20 |
jYCaMP: an optimized calcium indicator for two-photon imaging at fiber laser wavelengths.
Nature Methods. 2020 May 25;17(1):694-97. doi: 10.1038/s41592-020-0835-7
Femtosecond lasers at fixed wavelengths above 1,000 nm are powerful, stable and inexpensive, making them promising sources for two-photon microscopy. Biosensors optimized for these wavelengths are needed for both next-generation microscopes and affordable turn-key systems. Here we report jYCaMP1, a yellow variant of the calcium indicator jGCaMP7 that outperforms its parent in mice and flies at excitation wavelengths above 1,000 nm and enables improved two-color calcium imaging with red fluorescent protein-based indicators.