The GENIE Project Team at HHMI Janelia Research Campus have developed and characterized multiple transgenic mice expressing jGCaMP8s and jGCaMP8m (see https://doi.org/10.25378/janelia.24565837) and deposited the lines at The Jackson Laboratory (JAX).
Researchers now at Janelia generated over 18,000 driver lines (the VT collection) that exploit the GAL4, LexA, and split-GAL4 systems to express transgenes in distinct and highly specific cell types in Drosophila. In addition, approximately 1,000 of the most useful VT GAL4 lines are available from the Vienna Drosophila Resource Center.
The ability to reproducibly target the expression of transgenes to small, defined subsets of cells is a key experimental tool for understanding many biological processes. Unfortunately, the Drosophila nervous system has thousands of distinct cell types, and it has generally not been possible to limit expression to one or a few cell types with standard fly lines. Instead, intersectional methods can achieve the desired specificity by allowing expression of the transgene only where two different enhancers overlap in their expression patterns.
The data presented on the Split-GAL4 collection site are the work of the Janelia FlyLight Project Team, the Descending Interneuron Project Team, and the laboratories of Gerald M.
The data presented on this site are the work of the Janelia FlyLight Project Team and the laboratories of Gerald M. Rubin, James W. Truman, Richard S. Mann, and Christopher Q. Doe.
Researchers at HHMI Janelia Research Campus make popular transgenic zebrafish lines available to the scientific community through the Zebrafish International Resource Center (ZIRC).
Fly stocks generated in the Rubin lab are generally deposited in the Bloomington Drosophila Stock Center. For more specialized stocks please contact us directly.
In January 2011, the Rubin lab began depositing our collection of GAL4 driver lines in the Bloomington Stock Center at the rate of approximately 100 lines a week, and all lines were available for distribution by December 31, 2012. Data relating to these lines is available through the article and weblink (above and following):
http://www.sciencedirect.com/science/article/pii/S2211124712002926
