Imaging

The process of embedding tissue in the plastic resin dibutyl phthalate in xylene (DPX) creates optically transparent specimens and produces high-resolution, fluorescent confocal images.  To optimize resolution and make the entire thickness accessible at high magnification, the samples are mounted directly on a coverslip. DPX is applied to the samples before the coverslip is inverted onto a prepared slide.  The prepared slide supports the coverslip so that the tissue is not crushed and ensures uninhibited movement of short working distance microscope objectives.

Recent studies have provided compelling demonstrations that both mice and rats can be trained to perform various behavioral tasks while head restrained. To be effective for research studies, a system of rodent head restraint is required for electrophysiology and imaging, both in vitro and in vivo. The system must include stability, rapid customization, and rapid conversion between preparations and low cost.

Inventors at Howard Hughes Medical Center's Janelia Research Campus have developed a strategy for creating genetically encoded, intensity-based fluorescent sensors and an associated unique set of analytical tools that can measure the presence of a single molecular analyte in a complex mixture using standard optical technology. A series of Glucose Sensors (iGlucoSnFrs) made via this technique is also available.

Observing neural activity during behavior has become an important tool in understanding neural function. Behavior reflects nervous system activity and is dependent on multiple factors, including external stimuli, experience, neuronal structure, and changes in the internal milieu of the animal. Alterations at the cellular or functional level can profoundly alter basal and evoked activity.

Repeatable microscope observations at high magnification and resolution are difficult when using current slide holders for oil immersion due to the objective, oil, and slide movement on the microscope stage. Therefore, a unique microscope slide holder was designed to hold slides securely for oil immersion microscopy and other microscope systems that require a fixed slide position.