Imaging

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Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) is an advanced technique to make 3D images of biological cells in tissues with superior z-axis (vertical) resolution, generating easily interpretable data for reconstruction. FIB-SEM produces detailed images of cell features like protein complexes and membranes through an electron beam's sequential etching of a sample surface. Until the developments of Enhanced FIB-SEM by C. Shan Xu and Harald Hess, presented here, the image produced had been limited to a single cell or small volume of cells.

Although multiple electron microscopic techniques can achieve ~10 nm isotropic resolution, none are well-suited to image volumes at or above 1 mm³. Structural imaging at this scale opens new experimental achievements, like imaging large sections of vertebrate brains or an entire invertebrate nervous system. Unfortunately, current methods don’t meet the automatic resolution and throughput requirements needed to trace volumes spanning many cubic millimeters.

FISH Background

Overview

The JFX dye platform enhances the performance of Janelia Fluor® dyes by introducing deuterium at key positions, boosting brightness and extending photostability without altering their spectral signatures or cell permeability.

FLInChR is an engineered variant of a light-gated opsin, Channelrhodopsin, that functions as a potent optical inhibitor of neuronal activity.  Fusion to the ‘leader’ sequence results in the topological inversion of the opsin and converts it to a light-gated, non-specific cation pump. This ‘flipped’ opsin is efficient as an inhibitor of neuronal activity in both in vitro and in vivo. The ‘topological engineering’ of opsins used to generate FLInChR could serve as a robust platform to screen for variants of membrane-bound proteins with useful properties.

The temperature- and atmosphere-regulated sample chamber designed for the SiMView microscope developed in the Keller Lab is a substantial achievement in addition to the overall microscope innovation. Made to hold a specimen for four objectives, it can be replicated and modified for use with the SiMView microscope, for which designs are also available to researchers, or with other multi-view, multi-objective microscopes.

This technology is a non-gadolinium-based contrast material for use in MRI (magnetic resonance imaging) scans. Contrast material is injected into the patient to help visualize physiological structures and support the interpretation of images. Contrast materials in use today are only weakly influenced by magnetic and temperature changes in typical MRI magnetic fields. This novel contrast material is magneto-caloric, susceptible to sharp phase change at a range of direct current (DC) magnetic field values around physiological relevant temperatures.

MIMMS (Modular In vivo Multiphoton Microscopy System) is a modular platform for performing two‐photon laser scanning microscopy (TPLSM) optimized for in vivo applications. The system generally uses commercially available core parts for movement of the objective in the X‐, Y­‐, and Z­‐axis linear translation and X‐axis rotation for in vivo experiments. The backbone of the design is a movable, raised optical breadboard, providing a large area for affixing optical equipment associated with the microscope.

This page contains links to the template brain images and transformations described in Bogovic et al., "An unbiased template of the Dro