Imaging

There is a long history of putting a tethered insect on a ball to measure its movements in response to controlled sensory stimuli. As mentioned in the publication, Karl Götz and Erich Buchner had fly-on-a-ball systems working almost four decades ago. Our system uses modern image processing technology to acquire, with high temporal resolution, velocity about all axes of rotation of the ball. The first system to use optical mouse sensors came from Berthold Hedwig's lab, which used them to monitor crickets walking on a ball.

To obtain the documentation, designs, and parts list, please contact innovation@janelia.hhmi.org.

See additional explanations from the scientists here: https://www.janelia.org/lab/keller-lab/microscopes.

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Optical imaging of calcium dynamics using genetically encoded calcium indicators (GECIs) is a powerful tool for systems neuroscience. The current state-of-the-art GECIs emit green light (green GECIs) and are derived from Green Fluorescent Protein (GFP). Still, green fluorescence fails in several important experimental setups where tissue absorption is of concern, creating a need for red indicators.

Powerful Software Tools for Nanometer-Resolution EM Neuro-image Large Stacks' Segmentation

Image segmentation, a fundamental problem in computer vision, concerns the division of an image into meaningful constituent regions or segments.

GALA is a powerful, nano-resolution software tool developed to better understand the architecture and computation methods of the brain by analyzing and storing high-resolution neuroimaging stacks (such as those distributed under the open-source Janelia license, for example).

MIMMS (Modular In vivo Multiphoton Microscopy System) is a modular platform for performing two‐photon laser scanning microscopy (TPLSM) optimized for in vivo applications. The system generally uses commercially available core parts for movement of the objective in the X‐, Y­‐, and Z­‐axis linear translation and X‐axis rotation for in vivo experiments. The backbone of the design is a movable, raised optical breadboard, providing a large area for affixing optical equipment associated with the microscope.

This technology advances imaging techniques of biomicroscopy using an innovative type of a wide-field Multi-Focus microscope to enable fast, high-resolution 3D imaging.

These head plates have been used extensively in the Svoboda lab for in vivo imaging of the barrel cortex and other cortical areas (e.g., Huber et al., 2012). 
With this drawing, the end-user can have the Barrel Ctx Plate (Sloped Left) Head post (developed in the Svoboda Lab) machined at any respectable job shop. 

The Barrel Ctx Plate (Sloped Left) Head post, Head post with three epoxy holes, mounting arms, holders, and assembly by HHMI Janelia Research Campus (Karel Svoboda Lab) are licensed under free, non-commercial terms. 

The Lattice Light involves using a Bessel beam to illuminate a sample with light sheets that are sufficiently thin to achieve isotropic 3D resolution high-speed 3D fluorescent imaging. As a result, the technology can visualize cellular processes in spatiotemporal detail not attainable with other fluorescent microscopy techniques.

Maltose Sensor - Glutamate Sensor - Phosphonate Sensor

This PMT Amplifier is used as a current-to-voltage converter (transimpedance amplifier) for laser scanning microscopy. Depending on the FET OpAMP used, the bandwidth is up to ~100 Mhz, ideal for resonant scanning and well-matched to the ScanImage5 DAQ system.  In addition to the fast output, there is a slow output (1 kHz). The slow output is used to trip the PMT power supply (important for GaAsP and other sensitive detectors).

Opportunity: