This microscope images megapixel fields of view at over 1000 Hz within scattering tissue by scanning excitation lines at multiple angles and recovering images computationally. It can image large tissue volumes dramatically faster than other 2-photon microscopes.
Figure 1: Rendering of the SLAP Microscope system. A 3D pdf of the system above can be obtained here.
Drosophila melanogaster has a rich repertoire of innate and learned behaviors. Its 100,000-neuron brain is a large but tractable target for comprehensive neural circuit mapping. Only electron microscopy (EM) enables complete, unbiased mapping of synaptic connectivity; however, the fly brain is too large for conventional EM. Researchers led by the Bock lab developed a custom high-throughput EM platform and imaged the entire brain of an adult female fly at synaptic resolution.
CaMPARI (Calcium Modulated Photoactivatable Ratiometric Integrator) is a photoconvertible protein construct that enables imaging of the integrated calcium activity of large populations of cells over defined time windows. This second generation of CaMPARI molecules has several advantages over their predecessors. By mutating the amino acids in the photophore domain, researchers improved green brightness by 50% and red brightness by 250%.
Which GAL4 patterns are likely to yield useful genetic intersections that target your neurons of interest? Janelia researchers from the Scientific Computing Support Team, using images generated by the FlyLight Project Team, developed a method to improve the speed of querying large databases of fly lines to intersect.
The serotonergic system tracks the outcomes of actions to mediate short-term motor learning (Software)
This directs to a repository for the analysis algorithms used in Kawashima et al., Cell, 2016.
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