iAchSnFR: Acetylcholine Sensor
Overview:
A fast genetically encoded fluorescent sensor for faithful in vivo acetylcholine detection in mice, fish, worms, and flies
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cycleHCR: spatial mapping of RNA and Proteins
Mapping the locations of RNA in cells and tissues is a valuable technique in biomedical research for understanding fundamental principles and developing assays. Several spatial omics techniques have demonstrated the significance of that knowledge: hybridization methods such as MERFISH and seqFISH, along with sequencing methods like FISSEQ and STARmap, have led to remarkable discoveries.
HaloCaMP: Chemigenetic Calcium Indicators Using HaloTag and Janelia Fluor Dyes
Overview
HaloCaMP integrates circularly permuted HaloTag proteins with a range of Janelia Fluor (JF) dyes to form bright, modular calcium indicators for in vitro and in-cell imaging. This chemigenetic system offers far-red imaging, rapid labeling, and customizable readouts for neuroscience and cell biology.
Key Benefits
WHaloCaMP: Modular, Bright, and Multiplexable Chemigenetic Calcium Indicators
Overview
WHaloCaMP sensors integrate the high-performance Janelia Fluor dyes with genetically engineered HaloTag proteins for modular, tunable calcium imaging in living systems. This platform offers multiple dye options, works in live animals, and supports high-speed, multicolor, and fluorescence lifetime imaging. Fluorescence change in WHaloCaMP results from reversible quenching of the bound dye via a strategically placed tryptophan.
Biotin-Janelia Fluor® Conjugates
Overview
Biotin-Janelia Fluor® conjugates are cell-permeable, fluorescent ligands designed for affinity purification using the HaloTag platform with easy confirmation using imaging. Drug-JF compounds can elicit the translocation of proteins to defined subcellular regions.
BAPTA-JF: Bright and Targetable Ca²⁺ Indicators
Overview
BAPTA-JF indicators merge the superior properties of the Janelia Fluor® dyes, the Ca²⁺ sensitivity of BAPTA, and the genetic targeting precision of self-labeling tags like the HaloTag. This next-generation sensor platform delivers high-performance for both cellular and subcellular functional imaging.
Dyes with a Universal Coumarin Switch for Super-Resolution Microscopy
Overview
A versatile platform enabling the construction of dyes for super-resolution imaging by coupling a coumarin auxiliary to rhodamine dyes. Dyes can be tuned to be either photochromic, with 405 nm activation, or spontaneously blinking at physiological conditions, yielding a palette of dyes in different colors.
Bright, Photoactivatable (PA) Janelia Fluor® Dyes for Super-Resolution Imaging
Overview
Photoactivatable versions of Janelia Fluor® dyes combine the brightness and photostability of this small-molecule fluorophore platform with the spatial and temporal control of photoactivation, ideal for advanced imaging workflows.
Red-Shifted and Fluorinated Dyes "JF3" for Advanced Bioimaging
Overview
Fluorination and structural tuning of rhodamine scaffolds have yielded a powerful new class of bright, red-shifted fluorescent dyes for live-cell and super-resolution microscopy.
Spontaneously blinking JFb dyes
Overview
Applying the tuning strategies inherent in the Janelia Fluor® platform to hydroxymethyl (HM) derivatives of rhodamines yields a palette of spontaneously blinking dyes for super-resolution imaging. These dyes switch between fluorescent and nonfluorescent forms due to transient protonation/deprotonation allowing super-resolution microscopy without the need for redox buffers or strong illumination.