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Bootcamp Course: Optical Imaging Across Biological Length Scales

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Bootcamp Course: Optical Imaging Across Biological Length Scales

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July 28 - August 9, 2024
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Hosted by Teng-Leong Chew and Rachel Lee, this 2-week bootcamp course is designed to demonstrate how biological queries and hypotheses steer experimental designs on various microscopy platforms and across length scales from molecules to small animals.

Participants will receive didactic instruction and hands-on experience with localization super-resolution microscopy, structured illumination microscopy, STED, pixel-reassignment methods for enhanced resolution, expansion microscopy, and various light sheet microscopy methods. Participatory lab sessions will be conducted with biological samples from single cells to various model organisms, on commercial instruments as well as pre-commercial instruments developed at Janelia, including iPALM, MOSAIC, and SiMView. The course will put a strong emphasis on image processing and quantitative analyses using open source software, as well as proper methods for handling big data.

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Applications are open to anyone interested in understanding the full spectrum of optical imaging capabilities, including graduate students, postdocs, independent scientists, faculty and core facility managers. To be considered, applicants must justify their need to learn imaging on multiple modalities, expand on prior imaging experience, and explain their motivation for taking the course and how it will help their work. The course is designed to provide advanced training in optical microscopy. Prior microscopy experience is strongly encouraged. 

Janelia will cover the cost of accommodation and meals. Travel expenses are not covered, but support is available for those in need. Participants are expected to stay for the duration.

To be considered, applicants must apply online and provide:

  1. Research background and prior imaging experience.
  2. Brief statements on research interests and need for the course.

Applications are closed

Course Directors & Instructors

Teng-Leong Chew, HHMI/Janelia Research Campus
Rachel Lee, HHMI/Janelia Research Campus
Paul Tillberg, HHMI/Janelia Research Campus
Members of Janelia's Advanced Imaging Center and Integrative Imaging team.

Special Lectures

Luke Lavis, HHMI/Janelia Research Campus
Jennifer Lippincott-Schwartz, HHMI/Janelia Research Campus
Hari Shroff, HHMI/Janelia Research Campus
Ron Vale, HHMI/Janelia Research Campus


Interferometric PALM (Hess, Janelia)
Lattice Light Sheet microscope (Betzig, Janelia)
MOSAIC (Betzig, Janelia)
SiMView light sheet microscope (Keller, Janelia)
MuVi SPIM LS/CS/PM (Bruker)
Lightsheet 7 (Zeiss)
Lattice Lightsheet 7 (Zeiss)
Elyra 7 Lattice SIM  (Zeiss)
Expert Line STED (Abberior)
LSM 980 with AiryScan confocal microscope (Zeiss)
CSU-W1 SoRa spinning disk confocal microscope with TIRF (Nikon)